Dot-immunogold filtration assay for rapid screening of three fluoroquinolones
نویسندگان
چکیده
منابع مشابه
evaluation of dot immunogold filtration assay (digfa) by recombinant protein epc1 for anti- echinococcus granulosus igg antibody.
background: cystic echinococcosis is an important zoonosis caused by the echinococcus granulosus with a substantial impact in human and animal health in en-demic areas. the purpose of the present study was serodiagnosis optimizing of dog echinococcosis in order to achieve a rapid diagnostic method. methods: eight dogs were challenged with protoscoleces in order to have posi-tive echinococcosis ...
متن کاملEvaluation of Dot Immunogold Filtration Assay (DIGFA) By Recombinant Protein EPC1 for Anti- Echinococcus granulosus IgG Antibody
BACKGROUND Cystic echinococcosis is an important zoonosis caused by the Echinococcus granulosus with a substantial impact in human and animal health in endemic areas. The purpose of the present study was serodiagnosis optimizing of dog echinococcosis in order to achieve a rapid diagnostic method. METHODS Eight dogs were challenged with protoscoleces in order to have positive echinococcosis se...
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In the present study a new economic and rapid bacterial micro-assay for simultaneous detection and quantitative measurement of serum galactose was developed. Analysis of the standard curve showed a linearity range for galactose from 2 mg/dL to 180 mg/dL with a regression equation of Y = 0.013X ? 0.083; R² = 0.962. The advantage of the method is its ability to measure serum galactose quantitativ...
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A procedure is described for the routine laboratory diagnosis of viral serum antibodies. Antigens are dotted on nitrocellulose strips or sheets, and sera are applied on absorbent paper strips. Antigen-antibody complexes are detected with enzyme-conjugated antiglobulin and development of a colored, insoluble substrate product. The test allows processing of multiple sera in one 3- to 5-h operatio...
متن کاملA dot-immunobinding assay (dot-Iba) for rapid diagnosis of pulmonary tuberculosis.
IgG antibody to Mycobacterium tuberculosis from the sera of patients with 'definite' pulmonary tuberculosis (PT) was isolated and coupled with Cyanogen bromide-Sepharose 4B. Using an immunoabsorbent affinity chromatography, 14 kDa antigen was recovered from the culture filtrates of M. tuberculosis. With this mycobacterial antigen, a dot immunobinding assay (Dot-Iba) was developed for the detect...
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ژورنال
عنوان ژورنال: Food and Agricultural Immunology
سال: 2009
ISSN: 0954-0105,1465-3443
DOI: 10.1080/09540100902889936